Additional NGS DNA analysis of 484 genes, of which 38 (CCDC39, CCDC40, CCDC65, CCDC103, CCDC114, CCNO, CENPF, CFAP300, DAW1, DNAAF1, DNAAF2, DNAAF3, DNAAF4, DNAAF5, DNAH1, DNAH3, DNAH5, DNAH8, DNAH9 (= DNAL1), DNAH11, DNAI1, DNAI2, DNAJB13, DRC1, GDF1, LRRC6, MCIDAS, NEK4, NME8, PIH1D3, RSPH1, RSPH3, RSPH4A, RSPH9, SPAG1, STK36, TTC25, ZMYND10) potentially cause PCD, has shown a pathogenic homozygous sequence c.2014C > T in SPAG1 gene with autosomal recessive inheritance and thus causing PCD that results in male infertility [6]. This evidence concerns the gene DNAAF5 and primary ciliary dyskinesia.