In this study, we demonstrate: (i) Salmonella infection induces transient interactions between SCVs and peroxisomes; (ii) This interaction aids in the accumulation of cholesterol around SCVs necessary for SCV and SIF maintenance and bacterial growth, (iii) A bacterial protein in Salmonella, SseI contains a PTS1-like motif that mediates the interaction of SCVs with peroxisomes; (iv) SseI activates a host GTPase, ARF1, to induce PIP2 levels on peroxisomes, which facilitates the interaction of peroxisomes with SCVs using Syt7 as the tethering protein on SCVs (Schematic summary). The gene discussed is ARF1; the disease is Salmonella Infections.