SYT7 and Salmonella Infections: In this study, we demonstrate: (i) Salmonella infection induces transient interactions between SCVs and peroxisomes; (ii) This interaction aids in the accumulation of cholesterol around SCVs necessary for SCV and SIF maintenance and bacterial growth, (iii) A bacterial protein in Salmonella, SseI contains a PTS1-like motif that mediates the interaction of SCVs with peroxisomes; (iv) SseI activates a host GTPase, ARF1, to induce PIP2 levels on peroxisomes, which facilitates the interaction of peroxisomes with SCVs using Syt7 as the tethering protein on SCVs (Schematic summary).