To determine whether knockdown KLF4 in lung cancer cell lines could restore the stem-like properties loss caused by knockdown of MACC1, we conducted the rescue experiments by stably knocking down KLF4 in the shMACC1 stable cell line and control, described as shKLF4-shMACC1 and shKLF4-shNC, the expression efficiency was shown in Fig. 5E. The gene discussed is MACC1; the disease is lung carcinoma.