To test whether MACC1 regulate KLF4 via miR-25 and to determine whether the reduced expression of miR-25 was responsible for MACC1 silence mediated stabilization of KLF4 mRNA, we performed rescue experiments by transfecting miR-25 mimics in MACC1 stably knockdown in lung cancer cells. The gene discussed is KLF4; the disease is lung carcinoma.