After comparing the expression levels of MACC1 in common lung cancer cell lines (Fig. S1A), we used lentivirus infection to overexpress MACC1 in A549 cells with relatively low level (A549-pTRE3G-Tet3G-MACC1), and induced MACC1 depletion through short-hairpin RNA (shRNA) in NCI-H1299 cells with relatively high endogenous MACC1 expression abundance (H1299-pLKO-Teton-shMACC1). Here, MACC1 is linked to lung cancer.