When colon cancer cells were treated with OA, Asp and OA + Asp, a large number of cells were arrested in the S phase of the cell cycle, associated with increased expression of p53 and p21 proteins (Fig. 6C), which suggests that DNA damage, cell cycle checkpoint proteins were activated and consequently induced the cell cycle to undergo S-phase arrest. This evidence concerns the gene TP53 and malignant colon neoplasm.