Compared with AAV9-scramble (a scramble sequence) controls (NCAAV9), AAV9-Slc25a21 delivery enhanced Slc25a21 expression in renal tubules, tracked through IHC staining with SLC25A21-targeted HA (Fig. 2A, Fig. S2A), and partially restored the expression of Slc25a21 in AKI kidneys (Fig. S2B, C). The gene discussed is SLC25A21; the disease is acute kidney injury.