The reduction of fetal bovine serum (FBS) from 10% to 0% led to a dose-dependent decreased in intracellular PGRN protein levels in cervical cancer cells pretreated with 10% FBS (Fig. 2A), and addition of FBS from 0% to 10% enhanced PGRN protein levels in 0% FBS-pretreated cells (Fig. 2B). This evidence concerns the gene GRN and cervical cancer.