We treated a variety of melanoma cell lines (BrafWTNrasWT, BrafV600E, and NrasQ61R/L) with palbociclib for 1 day or 8 days, followed by a specific GLS1i (CB-839) for an additional 3 days to assess cell death by Annexin V staining, a well-established marker for apoptosis (Fig. 2A). Here, ANXA5 is linked to melanoma.