In this study, we assessed the expression of GLS1 in CDK4/6i-induced senescent melanoma cells and tested the applicability of targeting GLS1 to eliminate those senescent cells by GLS1 inhibition-mediated acidosis induction, given a recent study describing GLS1 as an essential gene supporting senescent cells for survival by neutralizing acidic conditions through ammonia production induced by upregulated GLS1 expression [37]. Here, GLS is linked to melanoma.