CD8A and infection: Given that trans-sialidase–specific CD8+ T cells did not recognize T. cruzi–infected cells within the first 48 hours after infection in previous research, the timing of our immunopeptidomics MHC-I profiling of T. cruzi at 48 post infection may have been too closely aligned with this timeframe, potentially contributing to the absence or limited availability of trans-sialidase peptides [60].