AKT1 and thromboangiitis obliterans: However, the results of the experiment performed by Mulvihill et al. [8] using mouse 3T3 cells overexpressing human IGF-1R to observe the effect of linsitinib on the Akt phosphorylation in the IGF-1R, PI3K/Akt, and ERK pathway differ from those of our experiment using the OFs of TAO patients.