However, while the experimental results obtained by Mulvihill et al. [8] showed that pretreatment with linsitinb also inhibited IGF-1-induced phosphorylation of Akt at Thr308 in mouse 3T3 cells overexpressing human IGF-1R, our results show that pretreatment with linsitinb did not inhibit IGF-1-induced phosphorylation of Akt at Thr308 in the OFs of TAO patients (Fig 4). The gene discussed is IGF1; the disease is thromboangiitis obliterans.