To understand whether the enzymatic domain of SIRT1 possess any role in mediating this interaction, we carried out NF-κB p65 immunoprecipitation in infected RAW 264.7 macrophages in the presence or absence of SIRT1 catalytic chemical inhibitor, EX-527 (1 μM) treatment at 16 hr post-infection. This evidence concerns the gene SIRT1 and infection.