Further, we estimated the production of another pro-inflammatory cytokine, IL-1β at 20 hr post-infection under the knockdown condition of Sirt1 and Sirt3 (Figure 3—figure supplement 1C) and observed heightened IL-1β production under knockdown of Sirt1 and Sirt3 in comparison to the scrambled infected control. Here, SIRT1 is linked to infection.