To evaluate the immune regulatory mechanism of SIRT1 in the S. Typhimurium (STM) infection scenario, we undertook SIRT1 immunoprecipitation in the infected RAW 264.7 macrophages at 16 hr post-infection alongside the uninfected macrophages and probed for NF-κB p65 interaction. The gene discussed is NFKB1; the disease is infection.