(C) Enumeration of GFP-positive bacterial cells through flow cytometry within F4/80-positive splenic macrophages present within splenic tissues homogenate harvested from adult male 6- to 8-week-old C57BL/6 mice (subjected to different chemical treatment – vehicle treated or SIRT1 [EX-527] or SIRT3 [3-TYP] inhibitor or SIRT1 activator SRT1720 treated at a dose of 1 mg/kg) on fifth day post STM infection. The gene discussed is SIRT1; the disease is infection.