(A) Enumeration of GFP-positive bacterial cells through flow cytometry in splenic tissues homogenate harvested from adult male 6- to 8-week-old C57BL/6 mice (subjected to different chemical treatment – vehicle treated or SIRT1 [EX-527] or SIRT3 [3-TYP] inhibitor or SIRT1 activator SRT1720 treated at a dose of 1 mg/kg) on fifth day post STM (expressing GFP) infection (107 CFU orally). This evidence concerns the gene SIRT3 and infection.