In this study, we infected Jurkat cells with pseudotyped virus HIV-1NL4-3-ΔEnv-GFP-Bcl2 to obtain a latent HIV-1 infection model that is more sensitive to R5M4 than to TNF-α (TNF-α is used as a reactivator in the construction of several HIV-1 latent infection cell models). This evidence concerns the gene TNF and HIV-1 infection.