C5 IL32+ TCs was mainly associated with leukocyte mediated cytotoxicity, leukocyte mediated immunity, cell killing, lymphocyte mediated immunity and regulation of T cell activation biological processes in GO-BP, and was associated with the natural killer cell mediated cytotoxicity, graft-versus-host disease, antigen processing and presentation, primary immunodeficiency and the Epstein-Barr virus infection pathways in KEGG. The gene discussed is IL32; the disease is graft versus host disease.