The results found that ECs highly expressed PLVAP, GNG11, SPARC, RGCC, IGFBP7; fibroblasts highly expressed CFD, LUM, DCN, GSN, MT2A; EPCs showed high expression of SPINK1, KRT19, LY6D, FXYD3 and S100A2; B plasma cells showed high expression of IGHG1, IGLC3, IGHA1, IGKC, IGLC2; myeloid cells showed high expression of HLA-DRA, CCL3, HLA-DPB1, C1QB and SPP1; bladder cancer tissue cells showed high expression of SPINK1, LY6D, KRT19, FXYD3, S100A2; paracancerous cells highly expressed genes including CFD, LUM, DCN, GSN, MT2A,RGCC and so on. Here, SPINK1 is linked to urinary bladder cancer.