To measure the proportion of internalized virions in SQSTM1/p62 + vesicles in ASFV-infected PAMs in the early phase of viral infection, the number of NHS ester-labeled ASFV in autophagosomes (labeled with SQSTM1/p62) and in endosomes (including RAB5 +EEs and RAB7 +LEs) was counted at 5, 15, and 30 mpi. The gene discussed is SQSTM1; the disease is viral infectious disease.