Lastly, to further decipher the combined functionality of circulating effector cells in melanoma patients following anti-PD1 treatment, cytokine secretion profiles were analyzed by ProcartaPlex dosages of PBMC supernatants after specific stimulation of each subset using alone or combined anti-CD3/CD28 antibodies, HMB-PP, IL-12/IL-18 and αGalCer. Here, CD28 is linked to melanoma.