Among three trajectory routes identified, we find that trajectory 2 represents a typical evolution route for the AST process: the tumor cells initially experience a transition from alveolar, glandular to basal identity, and then gradually lose stress responsive pathways (the interferon response and fatty acid metabolism pathways, key TFs such as CEBPA, CEBPB, NF-kB and RELA), and eventually reach the high proliferative state (enrichments of MYC and E2F targets). Here, NFKB1 is linked to neoplasm.