Recent advancements have introduced a refined BC method for generating cardiac tissues using Nkx2.5-Cre and Tie2-Cre with Rosa26-LoxP-STOP-LoxP-DTA (R26-DTA) systems,43 where Nkx2.5-Cre induces cardiac deficiencies and Tie2-Cre induces vascular deficiencies. This evidence concerns the gene NKX2-5 and breast cancer.