Recent advancements have introduced a refined BC method for generating cardiac tissues using Nkx2.5-Cre and Tie2-Cre with Rosa26-LoxP-STOP-LoxP-DTA (R26-DTA) systems,43 where Nkx2.5-Cre induces cardiac deficiencies and Tie2-Cre induces vascular deficiencies. Here, NKX2-5 is linked to hyperinsulinemic hypoglycemia, familial, 4.