We mainly used IVW as the method of MR analysis, a meta- analysis of IVW results in both cohorts showed that OPG was a protective factor for scoliosis (Folkersen 2020 – OR = 0.739, 95% CI = 0.611–0.893, P = .002; Zhao 2023 – OR = 0.833, 95% CI = 0.716–0.968, P = .017); however, no causal relationship was found between RANKL and RANK with scoliosis (RANKL OR = 1.048, 95% CI = 0.938–1.171, P = .411; RANK OR = 0.973, 95% CI = 0.871–1.087, P = .626) (Fig. 2). This evidence concerns the gene TNFRSF11B and scoliosis.