During pregnancy, we found significant increases in the mRNA levels of PIK3R1 (FC = 7.57; p < 0.001), SLC2A1 (FC = 1.61; p < 0.001), and SLC2A3 (FC = 4.01; p < 0.001), and a decrease in the expression of IRS1 (FC = 0.23; p = 0.046) in the GDM group vs. the NGT group. This evidence concerns the gene PIK3R1 and gestational diabetes.