The reduced invasiveness was also confirmed by the down-regulation of p-ERK2 and cyclin D1 expression in the cancer cells co-cultured with MDAPSF, as shown in the Western blot analysis in Figure 5B. The same experiments were performed for co-culturing the breast cancer cells in the presence of MDABM spheres, previously cultured for three days in the BM array (as schematized in Figure 5C). Here, CCND1 is linked to cancer.