En face ORO staining of whole aorta further confirmed a significant increase in atherosclerosis in male mice with NID2 overexpression [% plaque area: 12.5 ± 1.14 (74.09% increase)] compared with controls (7.18 ± 1.53), indicating a detrimental role for NID2 in the development of atherosclerosis (Figure 4B). The gene discussed is NID2; the disease is atherosclerosis.