Considering the important role of Cav1 in negatively regulating eNOS function in endothelial cells, and its direct functional impact on endothelial and SMCs function, we designed a study to investigate the effects of Cav1 on aortic wall endothelium and SMCs in a mouse model of MFS-associated aortic root aneurysms (FBN1+/Cys1041Gly) by generating male and female MFS mice lacking the Cav1 gene (MFS/Cav1KO). Here, NOS3 is linked to Marfan syndrome.