We conducted qRT-PCR to examine the expression levels of VWA8-AS1 in different squamous cell carcinoma cell lines including EBV-negative human skin squamous cell carcinoma (HSC1) cell lines compared against the EBV-positive HSC1 clone 1 (C1) and clone 6 (C6) cell lines, respectively. This evidence concerns the gene VWA8 and skin squamous cell carcinoma.