We conducted qRT-PCR to examine the expression levels of VWA8-AS1 in different squamous cell carcinoma cell lines including EBV-negative human skin squamous cell carcinoma (HSC1) cell lines compared against the EBV-positive HSC1 clone 1 (C1) and clone 6 (C6) cell lines, respectively. The gene discussed is VWA8; the disease is squamous cell carcinoma.