Since MMQ and AtT-20 cells, as functional pituitary adenoma cell lines, secrete prolactin (PRL) and adrenocorticotropic hormone (ACTH) respectively, we performed ELISA assays (Figure 3D) and found that the combination therapy also exhibited a synergistic inhibitory effect on hormone secretion in both cell lines. This evidence concerns the gene POMC and pituitary gland adenoma.