TYR and melanoma: Moreover, several synthetic derivatives were also proved to inhibit melanogenesis, e.g., (E)-1-(4-(3-chloro-4-fluorophenyl)piperazin-1-yl)-3-(4-methoxy-3-nitrophenyl)prop-2-en-1-one (Figure 6, 6a) is a mushroom tyrosinase inhibitor, and it inhibits tyrosinase activity in A375 human melanoma cells (at 25 and 50 μM) as well as melanogenesis in zebrafish in in vivo assays (at 25 and 50 μM) [117].