The expression levels of various proteins, specifically PIK3CB, AKT, pAKT, HDAC3, HDAC6, MMP9, MMP2, VIM, and α-SMA, which are implicated in hepatocyte degeneration as well as the initiation and progression of liver fibrosis, were analyzed using Western blotting. Here, ACTA1 is linked to Hepatic fibrosis.