VDR overexpression in conditioned medium stimulated M0 macrophages to differentiate into M2 lineage. VDR overexpression increased CCL20 levels in WT cells, but not in CCL20-promoter-knockdown ones. Using CCL20-neutralizing antibodies in the conditioned medium reduced M2 recruitment.Human PDAC tissue with higher VDR expression had higher M2/M1 ratio in both the tumor and stroma.In vivo, CCL20 blocking had antiproliferative effects and reduced M2/M1 ratio. The gene discussed is CCL20; the disease is neoplasm.