In a murine osteoblast cell line, zinc supplementation increases RUNX2 expression at the mRNA and protein levels, while zinc deficiency reduces RUNX2 expression and the nuclear RUNX2 protein levels during osteogenic differentiation induced by 10 mmol/L glycerol 2-phosphate and 50 μg/mL ascorbic acid [16]. The gene discussed is RUNX2; the disease is Zinc deficiency.