Figure 5B illustrates that the IBS group had considerably lower levels of IL-4 and IL-10 (p < 0.01) in comparison to the CON group, although there was a significant rise (p < 0.01) in IL-6 and TNF-α levels. Furthermore, in the IBS-induced animals, RNPs and RIF can raise IL-4 and IL-10 levels (p < 0.01) while lowering TNF-α and IL-6 levels (p < 0.05). This evidence concerns the gene IL10 and irritable bowel syndrome.