During spheroid formation, a small proportion of different cancer cell lines, such as colon cancer, cannot integrate into spheroids, and the loss of cell–cell adhesion molecules, such as E-cadherin, catenin, and P-cadherin, in SW20, DLD-20, and HCT116 cell lines is responsible for non-spheroid-forming phenotypes [28]. The gene discussed is CDH1; the disease is malignant colon neoplasm.