CREB1 and Alzheimer disease: Interestingly, a dual IF assay of CREB and HDAC2 (Figure 8A) supported by a negative Pearson correlation analysis (Figure 8B) indicated that upon treatment with 5 μM JRM-28 for 24 h, iPSC-derived AD neurons exhibited a strong upregulation of CREB coupled with a significant downregulation of HDAC2.