The ZO-1 area of untreated, HRV 16-infected cells was 44.39 ± 22.49% smaller after 48 h, and 44.02 ± 14.42% smaller after 72 h post viral infection compared to that of untreated, uninfected cells (48 h: -IFN-β1a/ + HRV 5130 ± 1235 μm2 vs. -IFN-β1a/-HRV 9794 ± 1965 μm2, p = 0.0148; 72 h: -IFN-β1a/ + HRV 4939 ± 810 μm2 vs. -IFN-β1a/-HRV 9177 ± 2075 μm2, p = 0.0376) (Fig. 2a, bottom right panel). The gene discussed is IFNA1; the disease is viral infectious disease.