Antibiotic treatment has been reported to be involved in intestinal and liver diseases by regulating multiple bile acid‐dependent transcriptional factors, including FXR, VDR, PXR, and so on.[8] We only observed decreased mRNA levels of Fxr and downstream gene Fgf15 in the ileum from vancomycin‐treated mice (Figure 2G), while no difference was noted in mRNA expression of Lxr, Pxr, Rorα, Tgr5, and Vdr (Figure S4, Supporting Information). This evidence concerns the gene NR1I2 and liver disorder.