Additionally, aortic sinus immunofluorescence revealed that SHep‐EV significantly reduced the expression of the smooth muscle cell contraction phenotypic marker α‐SMA, but increased the expression of the osteogenic marker RUNX2 (Figure 4H), indicating that SHep‐EVs could promote atherosclerosis and intimal calcification. Here, ACTA1 is linked to atherosclerosis.