NCF1 and infection: Primary infection of neutrophils with WT L. longbeachae resulted in ~22% decreased co-localization of p47phox to WT Lp-LCPs (ANOVA, P < 0.0001), but the reduced co-localization of p47phox to the WT Lp-LCP was dependent on the L. longbeachae T4SS (ANOVA, P > 0.05) (Fig. 6B and C).