TNF and intervertebral disk degenerative disorder: In the IDD group, the rats in the IDD group had severely impaired disk tissue, disordered cell arrangement, uneven pupanin coloring, and increased expression of TNF-α, IL-1β, IL-6, MMP-3, MMP-13, NLRP3 and Caspase-1, while collagen II and aggrecan levels were decreased.