SPI1 and leukemia: Based on the comparison of the differences in -Sign (P-value) * Log10(P-value) for these transcription factors between the leukemia cell lines and other types of cell lines, we selected six transcription factors with the highest differences between the leukemia and non-cancer groups for further experimental verification, namely ZNF460, SPI1, SPIB, ZNF384, ZNF784, and BATF3 (Figure 7B).