In parallel, we generated CPSF6 knock-out and non-targeting control primary CD4+ T cells and extracted RNA at 2 days post-electroporation to directly compare the transcriptional profiles between the infected cells at day 2 post-infection and CPSF6 knock-out cells at day 2 post-editing (immunoblot validation of knock-out efficiency in Extended Data Fig. 5b). This evidence concerns the gene CD4 and infection.