It has also been shown that even in the absence of a chemotherapeutic or cancer vaccine, TIM-4 blockade (anti-mouse TIM-4) combined with an antibody against programmed death-1 (PD-1) results in robust synergistic effects on tumor growth inhibition and overall survival in a syngeneic mouse model of colon cancer [19]. This evidence concerns the gene TIMD4 and colonic neoplasm.