Overall, these data demonstrate that NKG2DLs, but not DNAM-1Ls, are modulated during EBV infection; specifically, cell-surface MICB is expressed and can be released in a soluble form during latency and to a higher extent in cells with lytic EBV replication, whereas other NKG2DLs are very low or absent at the cell membrane in all phases of the viral life cycle, though ULBP2 and ULBP4 accumulate within cells in the lytic stage. Here, MICB is linked to Epstein-Barr virus infection.