Through H&E staining (Figure 2A) and multiplex immunofluorescence (mIF) staining (Figures 2B, C; Supplementary Figure S2A) of adjacent tissue sections, we found that the tumor cell-rich regions of the LG-ESS sample displayed positive CD10 expression (Figure 2B, right middle) and were located adjacent to smooth muscle cell populations (α-SMA+ DES+) (Figure 2B). Here, ACTA1 is linked to neoplasm.