Diet-induced NASH significantly increased serum ALT and AST levels (Figure 6A and B, Figure 6—figure supplement 1A and B), body weight, fasting blood glucose (Figure 6—figure supplement 2A, B, F, G), and liver fibrosis (Figure 6H and I, Figure 6—figure supplement 1H and I) in Slc39a5+/+ mice (Supplementary file 6). Here, SLC39A5 is linked to metabolic dysfunction-associated steatohepatitis.