MAPT and Alzheimer disease: However, an immunoblotting using an anti-MAPT antibody was conducted (Figure 3) to further validate the disease nature of these tissues, specifically AD, based on the hypothesis that MAPT aggregates (polymerization), commonly known as neurofibrillary tangles (NFTs), a hallmark pathological feature of AD brains (Binder et al., 2005), would be expected in the AD cortical tissues but not in the PD and CN tissues.