The real-time RT-PCR of the relative mRNA levels of the VEGF family (VEGF-A, VEGF-B, VEGF-C, and VEGF-D) for the Mary-X spheroids vs. the induced spheroids of other breast carcinoma cell lines revealed that the Mary-X spheroids expressed higher levels of both VEGF-C (p < 0.01) and VEGF-D (p < 0.05) compared to the induced spheroids of the other breast carcinoma cell lines (Figure 4A). This evidence concerns the gene VEGFC and breast carcinoma.