Our findings strongly supported that FMO3 inhibitor 3,3′-diindolylmethane (DIM), one of the key constituents of cruciferous vegetables (Sarkar et al., 2024), alleviates CSR-induced cognitive impairment by reducing TMAO level, upregulating astrocytic SREBP2 expression and brain cholesterol level, and finally reversing synaptic damage. Here, FMO3 is linked to Cognitive impairment.