NEFL and amyotrophic lateral sclerosis: In this study, using a cohort of newly diagnosed patients with ALS in Stockholm, Sweden, we aimed to (1) assess the relationship between various biomarkers commonly measured in clinical chemistry and survival in ALS; (2) investigate the synergistic associations of multiple biomarkers with survival, longitudinal functional decline, and level of NfL measured in cerebrospinal fluid (CSF); and (3) assess if biomarkers measured though clinical chemistry provide additional value to existing prognostic factors.