PCR analysis of the immunoprecipitated RNA showed that increasing Hotairm1 transcripts in early sepsis Gr1+CD11b+ cells increased the amount of S100 protein binding, whereas Hotairm1 knockdown in late sepsis Gr1+CD11b+ cells significantly reduced S100A9 binding (Figures 1C and 1D). This evidence concerns the gene ITGAM and Sepsis.