The expression of GSDMD, a key effector of pyroptosis, was evaluated in MCF-7 and MDA-MB-231 breast cancer cell lines following transfection with GFPT1-targeting siRNA (siRNA-GFPT1#3), an empty vector control (PcDNA3.1), and a GFPT1 overexpression vector (PcDNA3.1 (+)/GFPT1). The gene discussed is GFPT1; the disease is breast cancer.