We selected MHCC-97H, MHCC-97L, and HCC-LY10 cells to knock down NR4A3 and successfully established stably downregulated HCC cell lines (Fig. 3A and Fig. S2D) as well as NR4A3 knockout cell lines in MHCC-97H and MHCC-97L cells. The gene discussed is NR4A3; the disease is hepatocellular carcinoma.