Importantly, the ratio of Mtb-specific TRB clones in the blood, pleural effusion, or both of the PLTB patients is significantly higher compared with the health control (HC) group (Supplementary Excel Table S3), which suggests that the patients have the specific T-cell responses against Mtb, and the responses happened in both the blood and the pleural effusion (Figure 6I). This evidence concerns the gene THRB and Pleural effusion.