Cancer patients without treatment demonstrated the highest inhibition values (84.8 ± 5.2%), followed by patients with chemotherapy (79.8 ± 5.8%), positive estrogen receptor (76.4 ± 5.3%) and surgery + chemotherapy (71.4 ± 6.4%), etc. Inhibition values were independent of other parameters such as menopausal status, and smoking. The gene discussed is ESR1; the disease is cancer.